SIMULTANEOUS ESTIMATION OF SALBUTAMOL, AMBROXOL AND GUAIFENESIN IN TABLET DOSAGE FORMS BY USING RP-HPLC
Keywords:
Salbutamol, Ambroxol, Guaifenesin, Acetonitrile, potassium di-hydrogen phosphate buffer(pH-4)Abstract
A simple and reproducible method for simultaneous estimation of salbutamol (SAL), guaifenisin (GUA) and ambroxol (AMB) by high performance liquid chromatography (HPLC) in formulation was developed. The analysis was performed with a mobile phase containing acetonitrile (ACN) and potassium di-hydrogen phosphate (PDHP) adjusted to a pH- 4 in the ratio of 70 : 30 (% v/v) at a flow rate of 1.0 ml/min. A SHISEIDO C18 column (250 x 4.6mm i.d; 5μm) was used. A UV spectrum of salbutamol, guaifenisin and ambroxol was recorded by scanning between 200-400 nm, from the overlapping spectra a wavelength of 215 nm is selected and simultaneous estimation is carried out. The analysis was performed in the linearity range of 2-16 μg/ml for Salbutamol, 0.5-4 μg/ml for Ambroxol and 5-40 μg/ml for Guaifenisin respectively. The correlation coefficient was 0.996 (SAL), 0.994 (AMB), 0.998 (GUA). Thus the developed and validated chromatographic method for Salbutamol, Ambroxol and Guaifenisin is said to be rapid, precise (RSD<=2%), simple, accurate (% recovery=90-101%).
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