IN VITRO MICROPROPAGATION OF MEDICINAL PLANT OROXYLUM INDICUM-1
Keywords:
Oroxylum indicum -1, Zygotic embryos, cotylidanary nodes, Micropropagation, Seed, incubationAbstract
A simple and reliable protocol was developed through zygotic embryo culture and cotylidanary node explants of Oroxylum indicum -1 for multiple shoot regeneration. Effect of 3 cytokinins; BAP, KN and TDZ, was studied. BAP at 5mg/L proved better than KN & TDZ with highest frequency of shoot initiation and maximum number of shoots initiated.For zygotic embryo culture seeds were surface sterilized with 0.1% HgCl2, washed three times with sterile distilled water and were soaked for 24hrs. Seed coat was removed gently and isolated zygotic embryo (with cotyledons) and inoculated on ½ strength MS and MS basal medium with different levels of sucrose. Zygotic embryos were germinated within 7-8 days, cotylidanary nodes were germinated within 12 days of incubation.
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