ENUMERATION OF BACTERIA PRODUCING THERMOTOLARANT PROTEASES FROM COMMERCIAL MILK SAMPLES OF WARANGAL (AP)
Keywords:
Pasteurized milk, Bacillus sp, Thermotolerance, Proteases, Casein and GelatinAbstract
Proteases are the proteolytic enzymes present in all forms of life. Several microbial strains including fungi and bacteria have reported to produce proteases. Among proteases microbial proteases secure most important place due to their enormous industrial applications. The present investigations are amide to enumerate the bacteria isolated from milk samples of commercial brands available in Warangal urban market to produce thermotalerant proteases. The incidence of proteolytic bacteria is common in all brands of milk samples. The thermotolarant proteolytic activity varied among the isolates studied. Casein was found to be good substrate than gelatin for the production of proteases. The present studies showed that maximum activity of thermotolerant proteases was expressed at 50oC temperature.
References
1. Godfrey R. and M. West, Industrial enzymology, mac Millan publishers Inc, New York, 1996, 3-10.
2. Gupta R. O.K. Beg, and P. Lorenz,. APP. Microbiol. Biotechnol, 2002, 59, 15-20.
3. Anustrup K. Proteinases. In: Rose, A.H. (Ed.), Economic Microbiology. Microbial enzymes and Bicconvesion, Academic press, New York, 1980, 50-114.
4. Kuberan T. S.Sangaralingam and V. Tirumali Aragu, J.Biosci.Res., 2010.1(3), 163-174.
5. Wensing A.M. N.M.Van Maarseveen, M. Nijhuis, Antiviral Res, 2010, 85, 59-74.
6. de Oliveira L.O. C.A.Retamal, M. da Cunha, A.E. Oliveira, M.S. Pinto, K.V. Fernandes, Protein Pept. Lett. 2012, 19, 1281-1288.
7. Rawlings N.D. and A.J. Barrett, Families of serine peptidases, Methods Enzymol, 1994, 244, 19-61.
8. Khan F. H. Liu, A. Reyes, H.E. Witkowska, O. Martinez-Avila, L. Zhu, W. Li, S. Habelitz, Biochim. Biophys. Acta, 2012, 1830, 2600-2607.
9. Macfarlane R.G. Nature, 1964, 202, 498-499.
10. Ferrari E. A.S.Jarnagin and B.F. senmite, India.J. Microbiol., 1996, 42, 173-175.
11. Romero, E., J. Bautista, A.M. Garc ´a-Martinez, O. Cremendes and J. Parrado,. Process Biochem., 2007, 42, 1492-7.
12. Ferrero, M.A., G.R.Castro, C.M. Abate, M.D. Baigori and F. Singeriz,. Microbiol. Biotechnol., 1996, 45, 327-332
13. Valera, H., M.D. Ferrari, L. Belobradjic, R. Weyrauch and M.L. Loperena, World J. Microbiol. Biotechnol., 1996, 12, 643-645.
14. Hameed, A., T. Keshavarz and C.S. Evans, J. Chem. Technol. Biotechnol., 1999, 74, 5-8.
15. Gupta, R., Q.K. Beg, S. Khan and B. Chauhan, Applied Microbial. Biotechnol., 2002, 60, 381-395.
16. Phutrakul S. and Kansawud,. Sci. Fac, 1996, 23, 1-8.
17. Chi, Z., C. Ma, P. Wang and H.F. Li, Bioresour. Technol., 2007, 98: 534-538.
18. Gouda M.K. J.Microbiol, 2006. 55, 119-126.
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