DEVELOPMENT AND VALIDATION OF A NEW ANALYTICAL UV-VISIBLE SPECTROPHOTOMETRIC METHOD FOR THE ESTIMATION OF ALECTINIB IN API FORM AND MARKETED PHARMACEUTICAL DOSAGE FORM
Keywords:
UV-Spectroscopy, Alectinib, Method Development, Validation, AccuracyAbstract
Objective: The aim of this study is to develop three simple, specific and accurate spectrophotometric method for the determination of Alectinib in bulk and marketed pharmaceutical dosage form. Methods: In this method, the absorption spectrum of Alectinib is getting by 10μg/ml. In the developed method, the UV spectra were measured at 290 nm. Results: The proposed method was accurate, precise and selective for the determination of Alectinib in in pure form and in marketed pharmaceutical dosage form. Beer’s law was obeyed in the concentration range of 10–60μg/ml in this method. Conclusion: The developed method was used to determine the studied drug in bulk powder, laboratory prepared mixtures and pharmaceutical dosage form with good accuracy and precision. The method was validated according to ICH guidelines and the results obtained were statistically compared to those obtained from a reported method and were found to be in good agreement.
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