STUDIES ON IN VITRO ANTIOXIDANT AND ANTIBACTERIAL ACTIVITIES OF SPHAERANTHUS INDICUS (LINN)
Keywords:
Sphaeranthus indicus, Human pathogenic species, Antioxidant activity, Antibacterial activityAbstract
The present investigation was carried out for the evaluation of antioxidant and anti bacterial activities of leaf, flower, stem, root methnolic extracts of Sphaeranthus indicus. Antioxidant activity was conducted using various methods viz., DPPH, Hydroxyl radical, Nitric oxide, Superoxide anion and Hydrogen peroxide. Among the extracts tested flower and leaf extracts were exhibited highest scavenging activity at 150 μg/ml concentrations. Whereas, antibacterial activity was tested with human pathogenic species viz., Staphylococcus aureus, Bacillus subtilis, Bacillus cereus, Klebsiella pneumonia, Escherichia coli, Salmonella paratyphi. All the extracts tested produced minimum zones of inhibitions against all the species tested. The highest zone was exhibited by the flower extract of 8 against Staphylococcus aureus. The total phenolic content and flavanoid assay was carried to understand better relation with antioxidant activities of plant extracts.
References
1. Hamburger, M. and K. Hostettmann (1999). Free radicals in Biology and Medicine, 3rd ed. Oxford University Press, Oxford. 23-27.
2. Y.Z . Shu. Recent natural products based drug development: A pharmaceutical industry perspective, J. Nat. Prod, 1998, 61(8), 1053-1071.
3. M. Elwin-Lewis. Should we be concerned about herbal remedies. J. Ethanopharmacol, 2001, 75( 2-3), 141-164.
4. A.K. Goel, O.K. Kulshrestha, M.P. Dubey and S.M. Rajendran. Screening of Indian plants for biological activity part XVI. Ind. Journal of Experimental Biology. 2002, 40(7): 812-827.
5. K.R. Kirtikar, B.D. Basu Indian medicinal plants. 2nd ed. Dehradun: international Book Distributors;1987.
6. Gupta NS. The Ayurvedic system of medicine. Vol. II. New Delhi: Logas Press; 1984.
7. Paranjape P. Indian medicinal plants. In: Forgotten healer: A guide to Ayurvedic herbal medicine. Delhi: Chaukhamba Sanskrit Pratisthan:2001. P.148-9.
8. K. K .Baslas. Essential oil from Spharanthus indicus Pref Oil Rec, 1959, 50(X): 765-.
9. N.K. Basu, P.P. Lamsal. Chemical investigation of Sphaeranthus indicus Linn. Journal of the American Pharmaceutical Association 1946, 35(9), 274-275.
10. R.K.Gupta, S. Chandra, V. Mahandevan. Chemical composition of Sphaeranthus indicus Linn. Indian J Pharm 1967, 29(X), 47-48.
11. M.G. Gogate, L. Anathasubramanian, K.S. Nargund,. S.C. Bhattacharyya. Some interesting sesquiterpinoids from Sphaeranthus indicus Linn. (Compositae). Indian Journal of Chemistry 1986, 25(X), 233-238.
12. Shekhani MS, Shah PM, Yasmin A, Siddiqui R, Perveen S, Khan KM, et al. An immuno stimulant sesquiterpene glycoside from Sphaeranthus indicus. Phytochemistry. 1990,29(8),2573-2576.
13. R.N. Yadav and S. Kumar. 7-Hydroxy-3',4',5,6-tetramethoxy flavones 7-O-b-D-(1-4)-diglucoside, a new flavone glycoside from the stem of Sphaeranthus indicus. J Inst Chem, 1998, 70(X), 164-166.
14. V.L.Singleton and J.A. Rossi. Colorimetry of total phenolic substances. American Journal of Enology and Viti culture, 1965, 16(3), 144-158.
15. G.S.Kartashova and L.V. Sudos. Quantitative determination of flavanoids in the above-ground part of agrimony plants. Pharmaceutical chemical journal, 1997, 31(9), 488-491.
16. Ara. Naznin, and Nur. Hasan. In vitro Antioxidant activity of Methanolic leaves and flower extracts of Lippia Alba. Research Journal of Medicine & Medical Science, 2009, 4(1), 107-110.
17. S.M.Klein, G. Cohen, A.I. Cederbaumm. Production of form aldehyde during metabolism of dimethyl sulfoxide by hydroxyl radical generating systems. Biochemistry, 1981, 20(21), 6006-6012.
18. R. Govindarajan, S. Rastogi, M. Vijay Kumar. Studies on antioxidant activity of Desmodium of angeticum. Biological and Pharmaceutical Bulletin, 2003, 26(10), 1424-1427.
19. M. Nishikimi, N.A. Rao, K. Yagi.. The occurrence of superoxide anion in the reaction of reduced phenazine methosulfate and molecular oxygen. Biochemical and Biophysical research communications, 1972, 46(2), 849-854.
20. G.C. Yen and P.D. Duh. Scavenging activity of methanolic extracts of peanut hulls on free radical and active-oxygen species. Journal of Agricultural and Food chemistry, 1994, 42(3): 629-632.
21. G. Ilhami, A.A. Haci, C Mehmet. Determination of in vitro antioxidant activity and 15 radical scavenging activities of propofol. Chemical and Pharmaceutical Bulletin, 2005, 53(3):281-285.
22. C. Perez and M.P. Pauli. Bazevque. An antibiotic assay by the agar well diffusion method. Acta Biologiae et Medicine Experimentalis, Vol:15, 1990.
23. S.M.K. Rates. Plants as source of drugs. Toxicon. 2000, 39(2001), 603-613.
24. K.R. Kirtikar, Basu BD, ICS . Indian medicinal plants. In: Blatter E, Caius JF, Mhaskar KS, editors. 2nd ed. Vol-2. Allahabad India: Lalit Mohan Basu; 1981. pp. 1346–8.
25. A.K. Chakraborty, M. Behera, H. Roy, S. Rambhade. Sphaeranthus indicus Linn – a phytopharmacological review, International Journal of Drug Formulation & Research, 2010,1(3), 113-133.
26. C .Scanchez Moreno. Methods used to evaluate the free radicals scavenging activity in foods and biological systems, Food Science and Technology International, 2002, 8(3), 121-137.
27. T. kaneko, S. Tahara, M. Matsu. Retarding effect of dietary restriction on the accumulation of 8-hydroxy-2- deoxyguanosine in organs of Fischer 344 rats during aging. J Free Radic Biol Med, 1996, 23(1), 76-81.
28. A.E. Hagerman, K.M. RiedI, G.A. Jones, K.N. Sovik, N.T. Ritchard, P.W. Hartzfeld. High molecular weight plant polyphenolics (tannins) as biological antioxidants. J Agric. and Food Chem, 1998, 46(5): 1887-1892.
29. Halliwell, B., Gutteridge, J.M.C. 1985. Free radicals ageing and disease, free radicals in biology and medicine. 2nd ed. Oxford, UK.
30. I. Gqlcin, S. Beydemir, H.A. Alici, Y.M. Elmasta, M.E. Bqyqkokurolu. In vitro antioxidant properties of morphine. Pharmacol Res, 2004, 49(1), 59- 66.
31. J. Blaszczyk, J. Kedziora, M. Luciak, E. Sibinska, K. Trznadel, , Pawlicki, L. Effect of morphine and naloxone on oxidative metabolism during experimental renal ischemia and reperfusion. Exp Nephrol, 1994, 2(6): 364- 370.
32. Bedard, L., Young, M.J., Hall, D., Paul, T., Ingold, K.U. Quantitative study on the peroxidation of human low-density lipoprotein initiated by superoxide and by charged and neutral alkylperoxyl radicals. J Am Chem Soc, 2001, 123(50), 12439-12448.(search for full name of journal)
33. Rupesh Thakur, Rupal Singh, Nitika Jain. Evaluation of antibacterial activity of Sphaeranthus indicus L. leaves Journal of Pharmacy Research 2012,5(8), 4382-4388.
34. Brijesh K, Tiwari R.L, Khosa. Hepatoprotective and antioxidant effect of Sphaeranthus indicus against acetaminophen–induced hepatotoxicity in rats. J. Pharm. Sci. & Res, 2009, 1(2), 26-30.
35. Annie Shirwaiker, Kirti S, Prabhu, Punitha I.S.R. In vitro antioxidant activity of Sphaeranthus indicus (Linn). Indian journal of Experimental Biology, 2006, 44(12), 993-996.
36. Shahidi F, Natural antioxidants: An overview. In: Natural antioxidants, F., Shahidi. (ed.), AOCS Press, champaign, IL, pp. 1-11, (1997).
37. Kinuko Iwasa, Masataka Moriyasu, Yoko Tachibana, Hye-Sook Kim, Yusuke Wataya and Wolfgang Wiegrebe, Simple Isoquinoline and Benzylisoquinoline Alkaloids as Potential Antimicrobial, Antimalarial, Cytotoxic, and Anti-HIV Agents. Bio organic and Medicinal Chemistry, 2001, 9(11), 2871–2884.
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